Fans stormed the pitch at the Abuja National Stadium after Nigeria failed to qualify for this year's tournament - smashing up their own arena in shocking scenes
Then on Wednesday morning the Zambian FA confirmed Doctor Joseph Kabungo, who was acting as a doping officer at the game for the Confederation of African Football, had passed away while working.
A statement said: "The Football Association of Zambia joins the rest of the football family in mourning the late Dr Joseph Kabungo.
"Dr Kabungo, a CAF/FIFA medical officer was on duty in Abuja for the second leg, the final qualifying match between Nigeria and Ghana that ended 1-1."
President of the ZAF Andrew Kamanga did not reveal the cause of his death.
He said: "We take note that it is too early to dwell into the nitty-gritty of the cause of his death but will wait for the full report from CAF and FIFA on what exactly transpired.
"He was a dedicated and widely loved member of our football community and his impact was vast, having also been part of the 2012 Africa Cup-winning team.
"His death is a huge loss as Dr Kabungo was a friend and confidant to many generations of players and their families.
"He was one of the outstanding Zambian football personalities that flew the Zambian flag at global football events.
We all have to learn from his example of excellence and celebrate his legacy by breeding more hard-working football Zambian administrators."
Nigeria's 1-1 draw at home to Ghana saw the visitors qualify for the World Cup on away goals after a 0-0 draw in the first leg.
Ugly scenes ensued after the final whistle as the Nigeria supporters proceeded to storm the pitch and smash up their own ground.
The home fans clashed with police, who fired tear gas in an attempt to remove them, and tore down dugouts as their fury was channelled into violent acts in the stadium.
Ghana fans were subsequently moved onto the pitch after a number of them were reportedly attacked in the stands.
And the visiting players were forced to run for cover after being pelted with bottles.
